ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-0716-0190-7 ISBN 978-1-0716-0191-4 (eBook) © Springer Science+Business Media, LLC, part of Springer Nature 2020 This work is subject to copyright. Ralf Pörtner Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology (TUHH), Hamburg, GermanyĮditor Ralf Po¨rtner Institute of Bioprocess and Biosystems Engineering Hamburg University of Technology (TUHH) Hamburg, Germany
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Tested and trusted, comprehensive and reliable, all protocols from the series are indexed in PubMed. John Walker and constitute the key ingredient in each and every volume of the Methods in Molecular Biology series. These hallmark features were introduced by series editor Dr. Each protocol is provided in readily-reproducible step-bystep fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing.
Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, UKįor over 35 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Īnimal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.Īnimal Cell Biotechnology Methods and Protocols Fourth Edition The volume is divided into five parts that reflect the processes required for different stages of production. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Special emphasis was put on model-assisted concepts.
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Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production.
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